We propose to develop a microfluidic lab-on-a-chip device, assembling
and testing it.
Different laser processing methods will be applied in order to
integrate 2D and
3D polymer structures inside or at surface of photosensitive glass
micro-channels. Lowering the polymeric pattern dimensions to single
cell sizes
we aim at manipulating a single cell.
Besides different shaped patterns, polymers with variable composition
are
proposed by applying a recent implemented technique called
Combinatorial Matrix
Assisted Pulsed Laser Evaporation in view of guiding and trapping
single cells within a micro-environment created by laser processing.
Cancerous cells will
be cultivated on surfaces or in channels and their behavior tailored by
controlling the extensions of nano-space in local area or chemical
properties.
The chip will be connected to a pressure-based flow controller for
micro- and
nano-fluidics which enable a pulseless flow to mimic the in vivo
conditions
during a process live monitored and recorded under microscope.