We propose to develop a microfluidic lab-on-a-chip device, assembling and testing it. Different laser processing methods will be applied in order to integrate 2D and 3D polymer structures inside or at surface of photosensitive glass micro-channels. Lowering the polymeric pattern dimensions to single cell sizes we aim at manipulating a single cell. Besides different shaped patterns, polymers with variable composition are proposed by applying a recent implemented technique called Combinatorial Matrix Assisted Pulsed Laser Evaporation in view of guiding and trapping single cells within a micro-environment created by laser processing. Cancerous cells will be cultivated on surfaces or in channels and their behavior tailored by controlling the extensions of nano-space in local area or chemical properties. The chip will be connected to a pressure-based flow controller for micro- and nano-fluidics which enable a pulseless flow to mimic the in vivo conditions during a process live monitored and recorded under microscope.