The transfer by two laser methods
of four different types of protein biomolecules is proposed with the aim to
improve the osseointegration of biomimetic implants previously coated with a
nanometric layer of calcium phosphate also deposited by laser. The main
objective of this project is to cover calcium phosphate nanostructured coatings
obtained by Pulsed Laser Deposition on substrates of biomedical interest with
protein multistructures/multilayers or micropatterns by Matrix-Assisted Pulsed
Laser Evaporation. Biochemical assays will be corroborated with physicochemical
analyses, expecting a relevant feedback in developing and improving the
description of FTIR or wettability investigations after the biochemical
evaluation of the coatings. Finally, in vitro tests will verify the
functionality of the laser-transferred molecules versus bone marrow-derived
cells.