Nowadays biosensors have started to gain more and more attention in the field of health care. Advances in the biosensor design are appearing at a high rate as these devices play increasingly important roles in our daily lives. Surface Plasmon Resonance (SPR) biosensors are the most studied types of sensor at the moment. This project proposes a sensing structure faster than chemical detection that eliminates the need of chemical reagents and complex reactions. The biosensors will be made of an oxide waveguide covered with noble metal nanoparticles able to detect a chemical substance (analyte - cholesterol) or the end products of a reaction in which cholesterol is involved. In this project, transparent conductive oxide thin waveguides will be synthesized by Pulsed Laser Deposition (PLD). In a further step, noble metal nanoparticles  will be sputtered on their surface. Using the m-line optical interrogation method or spectrophotometric investigations, the response of the sensing structures to cholesterol will be studied. This project will find answers about the best match-ups oxide/metal in order to obtain the best detection resolution for cholesterol detection.