Miniaturized bio-platform for simultaneous melanoma combination therapy and tumorigenicity screening - MiniBioLab

Project number 131PED/2017
This contract is financed by
Romanian National Authority for Scientific Research,
Project code : PN-III-P2-2.1-PED-2016-1628

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Research Team

Coordinator - INFLPR

Institute of Biochemistry - Romanian Academy

Coordinator : Dr. Gabriela Chiritoiu
Dr. Cristian Munteanu


The goal of this project proposal is to design, develop and test the performances of a miniaturized bio-platform that will simultaneously address two objectives: 

    i) the synergistic co-targeting of melanoma cells by a precise combinatorial mixture of signaling path inhibitors (BRAFi, NRASi) and epigenetic modulators (HDACi); and

    ii) the evaluation of melanoma cells tumorigenicity guided by the geometric features of the patterns assembled on solid substrates.


Book chapters:

        1 “Recent Advances of Graphene Family Nanomaterials for Nanomedicine” Chapter, in “Fullerens, Graphenes and Nanotubes: A Pharmaceutical Approach” 1st Edition, Eds. Alexandru Grumezescu, Elsevier, ISBN 9780128136911 (2018). Irina NEGUT, Valentina GRUMEZESCU, Livia SIMA* and Emanuel AXENTE*

         2. “Combinatorial laser synthesis of biomaterial thin films: selection and processing for medical applications” Chapter, in “Advances in the Application of Lasers in Materials Science”, Eds. M. Dinescu. D.B. Geohegan, A. Miotello and P.M. Ossi, Springer (2018). Emanuel Axente, Carmen Ristoscu, Adriana Bigi, Felix Sima, and Ion N. Mihailescu


        1. “Biomimetic nanostructures with compositional gradient grown by combinatorial matrix-assisted pulsed laser evaporation for tissue engineering”  Under Review - Current Medicinal Chemistry (2018)  Emanuel Axente and Felix Sima 


        1. “High repetition rate UV versus VIS picosecond laser fabrication of 3D embedded microfluidic channels in photosensitive glass: comparative study” Under review - Nanomaterials (2018) F. Jipa, S. Iosub, B. Calin, E. Axente, F. Sima*, K. Sugioka*

        2. “Gradient multifunctional biopolymer thin film assemblies synthesized by Combinatorial MAPLE” Under review - Applied Surface Science (2018) Natalia Mihailescu, Merve Erginer Haskoylu, Carmen Ristoscu, Müge Sennaroglu Bostan, Mihai Sopronyi, Mehmet Eroğlu, Mariana Carmen Chifiriuc, Cosmin Catalin Mustaciosu, Emanuel Axente, Ebru Toksoy Oner, and Ion N. Mihailescu

        3. “ High speed picosecond laser processing for the fabrication of biomimetic cell-instructive environments” To be submitted to Applied Sciences (2018) Florin Jipa, Stefana Iosub, Emanuel Axente, Felix Sima and Koji Sugioka.

        4. „Combinatorial laser synthesis of compositional library thin films for screening of molecular-targeted agents in melanoma” To be submitted to ACS Combinatorial Science (2018) L.E. Sima,* I. Negut, V. Grumezescu, S. Iosub, G. Chiritoiu, C. Hapenciuc, F. Sima, and E. Axente,*.

        5. „Functionalized graphene oxide nanomaterials: in vitro cytotoxicity and BRAF inhibition in melanoma cells” FEBS JOURNAL Volume: 284 Special Issue: SI Supplement: 1 Pages: 328-328 Meeting Abstract: P.5.2-018 Published: SEP 2017 V. Grumezescu, I. Negut, S. Iosub, G. Chiritoiu, C. Hapenciuc, F. Sima, L.E. Sima,* and E. Axente,*

National and International Conferences:  

        1. “Mesoporous carbon films fabrication by Matrix-Assisted Pulsed Laser Evaporation”  Poster presented at Symposium X : Photon-assisted synthesis and processing of materials in nano-microscale of the E-MRS 2018 Spring Meeting, June 18-23, 2018, Strasbourg (France).  Emanuel Axente, Florin Jipa, Mihai Sopronyi, Camélia Matei Ghimbeu, Cristina Nita, Felix Sima.

        2. “Fabrication of three-dimensionalmicrofluidic channels in photosensitive glasses by picosecond pulsed laser”  Poster presented at the 19th International Symposium on Laser Precision Microfabrication (LPM 2018), June 25-28, 2018 in Edinburgh, Scotland, UK.  E. Axente,F. Jipa, S. Iosub, B. Calin,F. Sima,K. Sugioka.

        3. “Porous carbon thin films by Matrix-Assisted Pulsed Laser Evaporation from environmental friendly organic precursors” Poster presented at Symposium X : New frontiers in laser interaction: from hard coatings to smart materials of the E-MRS 2017 Spring Meeting, May 22-26, 2017, Strasbourg (France). E. Axente, M. Sopronyi, C.M. Ghimbeu, F. Sima.

       4. “MAPLE synthesis of Graphene Oxide composites for nanomedicine” Poster presented at IONS Balvanyos Conference 2017, International OSA Network of Students, 25-28 July 2017. I. Negut, V. Grumezescu, S. Iosub, C. Hapenciuc, F. Sima, G. Chiritoiu, L.E. Sima* and E. Axente*.

       5. „Functionalized graphene oxide nanomaterials: in vitro cytotoxicity and BRAF inhibition in melanoma cells” Conference: 42nd Congress of the Federation-of-European-Biochemical-Societies (FEBS) on From Molecules to Cells and Back Location: Jerusalem, ISRAEL Date: SEP 10-14, 2017 V. Grumezescu, I. Negut, S. Iosub, G. Chiritoiu, C. Hapenciuc, F. Sima, L.E. Sima,* and E. Axente,*


       1. „MiniBioLab Project: Current status and further perspectives”, Photonic Investigations Laboratory, CETAL Department, INFLPR, September 15, 2017. Emanuel Axente


        1. „Procedeu cu laser si plasma substractiv-aditiv pentru sinteza rapida de microstructuri 3D periodice din materiale organice si hibride pe substraturi solide” - Under preparation

Detailed Disemination

        1. The full Disemination report can be foud here.

Scientific Reports

    1. Scientific report for 2017 can be found here.

    2. Scientific report for 2018 can be found here